//Property: name
"name": "de-novo Transcriptome Assenmbly"

//Property: abstract
"abstract": "This document provides a comprehensive overview of de novo transcriptome assembly from RNA-Seq data, covering preprocessing, contamination detection, assembly methodologies, quality evaluation, and recent advances. It outlines best-practice workflows, popular tools (e.g., Trinity, rnaSPAdes), normalization strategies, and metrics for assessing completeness and accuracy. The material is structured as a teaching resource for practical and theoretical understanding of de novo RNA-Seq analysis."

//Property: author
"author":  [
  {
    "@type": "Person",
    "name": "Erwan CORRE"
  },{ 
    "@type": "Person",
    "name": "Mathieu GENETE"
  }
]

//Property: description
"description": "This document provides a comprehensive overview of de novo transcriptome assembly from RNA-Seq data, covering preprocessing, contamination detection, assembly methodologies, quality evaluation, and recent advances. It outlines best-practice workflows, popular tools (e.g., Trinity, rnaSPAdes), normalization strategies, and metrics for assessing completeness and accuracy. The material is structured as a teaching resource for practical and theoretical understanding of de novo RNA-Seq analysis."

//Property: audience
"audience": {
    "@type": "Audience",
    "audienceType": "beginner in high-throughput sequencing data analysis, starting at graduate level" 
}

//Property: educationalLevel
"educationalLevel": "Beginner"

//Property: inLanguage
"inLanguage": ["en-US"]
  
//Property: teaches
"teaches": [
    "The student will be able to understand the complete workflow for de novo transcriptome assembly from RNA-Seq reads.", 
    "The student will understand how to evaluate assembly quality using metrics (N50, ExN50), read-mapping statistics, and BUSCO/DOGMA."
]

//Property: keywords
"keywords": "RNA-Seq, de novo assembly, contamination, bioinformatics, quality assessment"

//Property: learningResourceType (presentation or handout)
"learningResourceType": ["presentation"]

//Property: license
// "https://creativecommons.org/licenses/by-sa/4.0/"
// "https://mit-license.org/"
"license": ["https://creativecommons.org/licenses/by-sa/4.0/"]

//Property: dateModified
"dateModified": "2025-11-17T0:00:00+00:00"